氫氣治療大鼠心肌梗死

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這個文章在6月12日被接受。是我們的第6篇，是世界上的17篇文章。目前全世界這個領域已經達到18篇論著。

文章採用最常規的方法：製備心肌梗死動物模型（這個比較麻煩一些），給氫鹽水，觀察功能、梗死體積和細胞損傷等常見指標，結合氧化損傷指標測定。

而且這個方面日本已經有論文發表，我們新在給氫方式上，審稿專家給的意見比較好。可惜雜誌的發表週期很長，否則早就發表了。我們是去年的實驗。

到目前我們已經發表了4篇採用注射方法的論文，也是目前唯一發表的4篇。

氫氣飽和鹽水可保護（大鼠）心肌的缺血/再灌注損傷

摘要

此前的研究表明，H2在心肌的缺血/再灌注損傷中具有保護作用。本研究旨在驗證如下假設――價廉物美、操作簡單的氫氣飽和鹽水可保護大鼠心肌受缺血（30分鐘）/再灌注（24小時）所致的損傷。成年雄性SD大鼠夾閉左冠狀動脈30分鐘/再灌注24小時。在再灌注前，腹腔注射氫氣飽和鹽水後能明顯降低大鼠血漿及心肌中的丙二醛（MDA）濃度、減少心肌細胞的凋亡和降低AAR區的8羥基鳥嘌呤核苷水準、抑制caspase－3的活化和減少梗死面積。再灌注24小時後，包括LVSP、LVDP的+(dP/dt)及－(dP/dt) 最大值等反映心功能的參數也明顯改善。結論：（給藥）氫氣飽和鹽水是一種新型、簡單、安全、有效的緩解心肌缺血/再灌注損傷的方法。

關鍵字：再灌注，氧化應激，富含H的鹽，凋亡

Hydrogen-rich saline protects myocardium against ischemia/reperfusion injury in rats
Qiang Sun 1, Zhimin Kang 1, Jianmei Cai 1, Wenwu Liu 1, Yun Liu 1, John H Zhang 2, Petar J Denoble 3, Hengyi Tao 1, and Xuejun Sun 1*
1 Second Military Medical University
2 Division of Neurosurgery, Loma Linda University, Loma Linda, California, USA
3 c Divers Alert Network-an associate of Duke University Health System

* To whom correspondence should be addressed. E-mail: sunxjk@hotmail.com.

Abstract

Protective effect of hydrogen (H2) gas on cardiac ischemia-reperfusion (I/R) injury has been demonstrated previously. This study was designed to test the hypothesis that hydrogen-rich saline (saline saturated with molecular hydrogen) which is easy to use induces cardioprotection against ischemia (30 min) and reperfusion (24 h) injury in rats. Adult male Sprague-Dawley rats underwent 30 minute occlusion of the left anterior descending (LAD) coronary artery and 24 hour reperfusion. Intraperitoneal injection of hydrogen-rich saline before reperfusion significantly decreased plasma and myocardium malondialdehyde (MDA) concentration, decreased cardiac cell apoptosis and myocardial 8-hydroxydeoxyguanosine (8-OHdG) in area at risk zonesAAR, suppressed the activity of caspase 3, and reduced infarct size. The heart function parameters including left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVDP)+(dP/dt)max and -(dP/dt)max were also significantly improved 24 h after reperfusion. It is concluded that hydrogen-rich saline is a novel, simple, safe and effective method to attenuate myocardial I/R injury.

Key Words: reperfusion, oxidative stress, hydrogen-rich saline, apoptosis

全文


本文引用地址：http://blog.sciencenet.cn/blog-41174-238354.html

資料來源 http://blog.sciencenet.cn/blog-41174-238354.html

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