Wechat ID： a0919730053
這個領域正想我2年前估計的那樣，開始快速發展了，這個研究是針對人類一個非常普遍的疾病：結腸炎，這個疾病不厲害，但嚴重困擾許多人的生活。有這個病的人都有體會。而且治療手段非常缺乏。主要是病因不清楚。但炎症反應是非常確定的。氫治療該疾病只針對炎症，雖然不能針對病因。但也提供了一個非常值得考慮的方法。這個研究非常值得重視。預示著氫分子醫學向更廣泛領域的發展。而且更具有實際意義。Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate
Received 18 May 2009.
Available online 30 May 2009.
AbstractBy its antioxidant effect, molecular hydrogen gas (H2) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5%), (2) DSS (5%) and H2, or (3) H2 only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12, TNF-α and IL-1β in colon lesion, were significantly suppressed by the addition of H2 to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H2. Therefore, the present study indicated that H2 can prevent the development of DSS-induced colitis in mice.
Keywords: Molecular hydrogen; Colitis; Inflammation; Antioxidant; Colon; Dextran sodium sulfate; IL-12; TNF-alpha; IL-1-beta; Macrophages
Fig. 1. Effects of H2 on the clinical features of DSS-induced colitis in BALB/c mice. Temporal change of H2 concentration in stomach (A) and in colon (B) after the oral administration of H2-saturated water to mice (n = 5/each time point) was monitored. Distilled water saturated with H2 (0.78 mM, ORP = −511 mV, pH 7.67) was applied to mice using a Popper® feeding needle (1 ml/mouse). Immediately after the sacrifice of animals in each group, H2 concentrations of liquid substance present in stomach and colon were measured. To examine the effects of H2 on DSS-induced colitis, H2 (0.78 mM) water alone or 5% DSS with or without H2-water were administered to mice (8 w males, n = 6/group) ad libitum for 7 days. The percent of body weight change (C) and colitis score (D) were measured on a daily basis. Data points and bars of H2-water alone (♦), 5% DSS water alone (□), and 5% DSS including H2-water () indicate means ± SD. *p < 0.05, **p < 0.01: Value is significantly different from the control group receiving DSS alone in the same measurement day (t-test).
Fig. 2. H2-mediated alleviation of colon contraction induced by DSS. To assess the severity of colitis, regular water, H2 (0.78 mM) water alone, or 5% DSS with or without H2-water were administered to mice for 7 days after which the colon length was measured. (A) Representative macroscopic features of the colons isolated from mice treated with regular water (Control), H2-containing water (H2), 5% DSS water (DSS) and 5% DSS containing H2-water (H2 + DSS) are shown. (B) The measurement of mean ± SD of colon length in each group at Day-7 is depicted. **p < 0.01: Value differs significantly between the groups indicated by brackets (t-test).