終於等到全文和正式頁碼The Effects of Hydrogen-Rich Saline on the Contractile and Structural Changes of.pdf
Journal of Surgical Research, Volume 167, Issue 2, 15 May 2011, Pages 316-322Han Chen, Yan Ping Sun, Ping Fang Hu, Wen Wu Liu, Hong Gang Xiang, Yang Li, Rong Lin Yan, Ning Su, Can Ping Ruan, Xue Jun Sun, Qiang Wang
Methods. Intestinal I/R injury was induced in Sprague-Dawley rats using bulldog clamps in superior mesenteric artery by 45 min ischemia followed by 1 h reperfusion. Rats were divided randomly into four groups: sham-operated, I/R, I/R plus saline treatment, and I/R plus hydrogen-rich saline treatment groups. Hydrogen-rich saline ( > 0.6 mM, 6 ml/kg) or saline (6 ml/kg) was administered respectively via tail vein in 30 min prior to reperfusion. Following reperfusion, segments of terminal jejunum were rapidly taken and transferred into isolated organ bath and responses to KCl were recorded. Samples of terminal jejunum were also taken for measuring malondialdehyde and myeloperoxidase. Apoptosis in intestinal epithelium was determined with terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling technique (TUNEL). Expression and distribution of proliferating cell nuclear antigen (PCNA) were detected with immunohistochemistry.
Results. Hydrogen-rich saline treatment significantly attenuated the severity of intestinal I/R injury with inhibiting I/R-induced apoptosis and promoting enterocytes proliferation. Moreover, Hydrogen-rich saline treatment significantly limited the neutrophil infiltration, lipid oxidation and ameliorated the decreased contractility response to KCl in the intestine subjected to I/R.
Conclusions. These results suggest that hydrogen treatment has a protective effect against intestinal contractile dysfunction and damage induced by intestinal I/R. This protective effect is possibly due to its ability to inhibit I/R-induced oxidative stress, apoptosis and to promote epithelial cell proliferation.
Keywords: Hydrogen, Intestinal ischemia/reperfusion, Antioxidant, Oxidative stress, Contractility, Apoptosis